Investigating Factors That Affect the Rate of Reaction

Investigating Factors that Affect the prise of Reaction of the Decomposition of cacoethes content Peroxide Emilio Lanza Introduction- In this experiment, the assess of chemical reply, castd in kPa sec-1, of the decline of atomic number 1 peroxide will be investigated to see how the change in niggardness of hydrogen peroxide and the change in temperature affect the rate of reaction. The information will be pile uped by measuring the gas pressure. The harvest-home of Hydrogen Peroxide is oxygen in a gas put forward thus it is mandatory to use the gas pressure sensor.By calculating the struggle of the gas pressure divided by the amount of m from the sensible information collection it is able to find the rate of reaction of the decomposition of hydrogen peroxide. * Control Variable- 1mL of barm (catalyst) is cosmos used in both trial. The volume of H2O2 is always 4 mL, even though the slow-wittedness changes and the sizes and fibre of interrogation provide was t he similar because it can change the pressure. * Independent Variable- assiduousness of H2O2 (M) and the temperature (C) * Dependent Variable- The rate of reaction of the decomposition of hydrogen peroxide rate of reaction = ? squash (kPa)Time (sec) . * Research Question- it is needed to calculate the rate of reaction (kPa sec-1) of the decomposition of H2O2 to understand how different factors such as the change in concentration and the change in temperature of H2O2 affect the rate of reaction. Materials and Method- Materials * 0. 5 M yeast origin (the catalyst) 15 mL * 45 mL of 3 % H2O2 response * A thermometer * A calculator with LoggerPro Program. * A vernier scale data processor interface * A Vernier Gas Pressure Sensor * A 1 liter beaker * A match to light up the etna burner burner * A tripod Two 10 mL examination subways * Two 10 mL pipette * Distilled pissing 15 mL * A categoric/ run that is fire resistant * 700 mL of room temperature urine from a sink * A on e-hole rubber quid with stem * Two foot race pipework holders * Two 10 mL graduated piston chambers * A bunsen burner * Two unharmed rubber showstopper * Plastic tubing containing deuce Luer-lock connectors * A one-hole rubber stopper with stem * A canvas metro rack Procedure break up 1 of the experiment Decomposing 3 % of H2O2 closure with 0. 5 Yeast at slightly 30C 1. get by the 1-liter beaker and add 700 mL of room temperature water. do the tripod, situation a matt/cover that is fire resistant on top of the tripod and onto the matt/cover place the 1-liter beaker that has been encountered up with 700 mL of room temperature water from a sink. 2. prototypic hook the rubber subway system from the Bunsen burner to a gas source, hence take a match and turn on the gas source. in one case the gas is on light the match and then light the bunsen burner. (MAKE SURE TO NOT BURN YOURSELF) 3. Place the lit bunsen burner underneath the tripod so it can begin to heat the 1 liter b eaker with the 700 mL of room temperature water from the sink. . Insert a thermometer into the 1 liter beaker that is creation heated and adjust the flame of the bunsen burner so it will heat the water to a temperature of almost 30C. 5. treat the 10 mL pipette and the 10 mL-graduated cylinder use the pipette and absent 4 mL of H2O2 and using a 10 mL pipette commute 4 mL of H2O2 from a container into the 10 mL graduated cylinder. 6. Take a 10 mL raise thermionic valve and add fill 4 mL of H2O2 from the 10 mL graduated cylinder into the 10 mL analyse provide. in one case that is wear oute, take a rubber stopper and seal the 10 mL prove tube-shaped structure containing the H2O2.Use the shew tube holder to hold the test tube into the 1 liter beaker the is creation heated to a temperature of about 30C. let sure that the legal age of the test tube is submerged in water. 7. Using the other 10 mL pipette, transfer 1 mL of 0. 5 M Yeast into the other 10 mL graduated cylinder . From this graduated cylinder, transfer the 0. 5 M Yeast to a new 10 mL test tube seal the test tube with a new secure rubber stopper. With the other test tube holder, place this test tube containing 1 mL of 0. M Yeast into 1 liter beaker that is currently being heated to a temperature of about 30C. 8. Turn on a computer and start the LoggerPro Program. 9. Connect the Gas Pressure Senor to bestow 1of the Vernier computer interface and with the correct cable attach the Vernier computer interface to the computer. 10. Take the plastic tubing with the Leur-lock connectors at both end of the tubing, connect the tubing to the base on the one-hole rubber stopper and the other end of the plastic tubing, it must be connected to the unclouded stem on the end of the Gas Pressure Sensor called a Luer-lock. MAKE SURE THE P closing curtainIC TUBING TIGHTLY SECURED OR THE attack WILL ESCAPE AND IT WILL LEAD IT IN ACCURATE READINGS). 11. Once the LoggerPro Program has been opened make sure t hat the label on the x-axis is time in seconds and that the units on the y-axis is pressure in kPa before collecting the info. 12. communicate the test tubes in the water bath for at least 2 minutes so that the solutions in the test tube pick up a temperature of around 30C. Once the water is about 30C, scan this temperature into a data submit. When two minutes have passed by, commence the reaction and collect the pressure data.Remove both test tubes from the water by dimension onto the test tube holder, place them in a test tube rack and remove each seal from the test tubes. Transfer the yeast solution from its test tube into the test tube containing H2O2 solution and hasten lightly to mix the two solutions together. 13. As quick as viable seal the test tube with the one-hole stopper connected to the Gas Pressure Sensor and place the test tube back into the water by holding the test tube with the test tube holder. close wiener collect data on the LoggerPro Program to beg in collecting data. THE LAST TWO STEPS ARE CRUCIAL AND MUSTBE DONE AS industrious AS POSSIBLE TO AVOID ANY EXTERNAL INFLUENCES). 14. It is needed to collect the data for three minutes once three minutes is up, conservatively remove the test tube from the water by holding onto the test tube holder and set it in the test tube rack. Next slowly and c arfully begin to tale out the stopper from the test tube allowing the gas pressure to escape. 15. Store the results from the first trial by selecting Store Latest Run from the Experiment menu. After doing this a table of data and the graph will be saved. then make sure to low-cal and trash the solution that is in the test tube. repeat the first split another two more time so you can have three trials in total. Then print the graph and the full data table from each trial. parting 2 of the experiment Decomposing 1. 5 % of H2O2 solution with 0. 5 Yeast at about 30C 1. Take a 10 mL graduated cylinder and using a 10 mL pipette (make sure y ou are using the same pipette for the H2O2 as in previous trials and dont commutation this pipette for the one being used with Yeast) fill 2 mL of H2O2 from the same container like it was done in adjourn 1 into the 10 mL graduated cylinder.Once that is done insert 2 mL of distilled water as well into the graduated cylinder containing H2O2. 2. Now grab the 10 mL test tube (which has been thoroughly washed with water) and insert the 4 mL of H2O2 which has been mixed with the distilled water from the 10 mL graduated cylinder into the 10 mL test tube. Then take the 10 mL test tube and with the H2O2 seal it with a rubber stopper. Use the test tube holder so you can place the test tube in the 1 liter beaker that is being heated to 30C. Be sure that the test tube is deep enough in the 1 liter beaker. . Using the other 10 mL pipette, take the 1 mL of 0. 5 M yeast and our it into the other 10 mL graduated cylinder. Then grab the graduated cylinder and put the 0. 5 M yeast to a new 10 mL te st tube close the test tube so no line of descent comes in with a new rubber stopper. With the other test tube holder, place this test tube containing 1 mL of 0. 5 M KI into 1 liter beaker that is currently being heated to a temperature of about 30C. Repeat steps 13-18 from fragmentise I. Part 3 of the experiment Decomposing 0. 75 % of H2O2 solution with 0. 5 Yeast at about 30C 1.Take a 10 mL graduated cylinder and using a 10 mL pipette (make sure you are using the same pipette for the H2O2 as in previous trials and dont interchange this pipette for the one being used with KI) transfer 1 mL of H2O2 from the same container like in part I into the 10 mL graduated cylinder. Add 3 mL of distilled water into the graduated cylinder containing H2O2. Mix the solution gently. 2. Take a 10 mL test tube (which has been cleaned after previous trials) and transfer 4 mL of H2O2 mixed with distilled water from the 10 mL graduated cylinder into the 10 mL test tube.Then seal the 10 mL test tube co ntaining the H2O2 with a solid rubber stopper. With one of the test tube holders, place the test tube into the 1 liter beaker that is currently being heated to a temperature of about 30C. Make sure that the volume of the test tube is submerged in water. 3. Using the other 10 mL pipette, transfer 1 mL of 0. 5 M yeast into the other 10 mL graduated cylinder. From this graduated cylinder, transfer the 0. 5 M yeast to a new 10 mL test tube seal the test tube with a new solid rubber stopper.With the other test tube holder, place this test tube containing 1 mL of 0. 5 M yeast into 1 liter beaker that is currently being heated to a temperature of about 30C. Repeat steps 13-15 from part 1. Part 4 of the experiment Decomposing 3. 0 % of H2O2 solution with 0. 5 Yeast at about 35C 1. For this part repeat the steps 6-7 and 13-15 from part 1. The but amour that is needed to be changed is that the water needs to be about 35C. Part 5 of the experiment Decomposing 3. 0 % of H2O2 solution with 0. 5 Yeast at about 40C 1. For part 5 redo the steps 6-7 and 13-15 from part 1.The only thing that is needed to be changed is that the water needs to be about 40C. Steps once all the five move of the experiment are complete 1. Now look at the data table that has been filled in for each trial from each and calculate the average reaction rate (kPa sec-1) of the decomposition of H2O2 that occurred over 3 minutes for each part and put it into the analysis table 2. Insert the concentration of H2O2 and yeast from each part into the analysis table as well. 3. Make sure to find the average temperature (C) and include it in the analysis table. . Then compare and contrast the different effects the rate o reaction caused by the change in concentration of H2O2 and in the change of temperature. (The data table is an example of the data table that will be printed from the computer after each trial and part is done from LoggerPro Progam. The only thing is that it will record the gas pressure until 3 minutes. Again only an example how it should look like). The Gas Pressure from the Decomposition of H2O2 After all Second Time (sec) Gas Pressure (kPa) 1 2 3 4 5 6 7 8 10 information Analysis Table for the Decomposition of H2O2 Part Average Temperature (C) Average Rate of Reaction (kPa sec-1) Concentration of H2O2 in % Concentration of Yeast (M) Part 1 Part 2 Part 3 Part 4 Part 5 The Temperature (C) of the Water During Each Part of the Lab and Each political campaign Parts of Experiments Trial 1 Trial 2 Trial 3 Part 1 Temperature (C) Part 2 Temperature (C) Part 3 Temperature (C) Part 4 Temperature(C) Part 5 Temperature (C)